Almost all scientific institutions use chromatographic methods in their activities. Because the main carriers of scientific knowledge are concentrated in research, academic, technological, design institutes and various laboratories.The process of interaction of the analyzed sample components with the stationary phase is often not fully known to the scientific researcher providing chromatographic analysis. Theoretical knowledge of the substances separation is common to all types of chromatography.The substances separationoften doesn’t depend on the type and method of processing chromatograms and practical methods of chemical analysis.It is necessary to keep these aspects in mind when optimizing known and developing new chromatographic analysis techniques.
